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激光辐照对湛江等鞭金藻的复壮作用及机制研究
光生物学 湛江等鞭金藻 Nd∶YAG激光
2009/8/13
用Nd∶YAG激光（波长1.06 μm,功率密度6 W/cm2）对长期保存的湛江等鞭金藻藻种进行辐照处理.研究了激光辐照对藻生长和生理生化特性的影响，并从藻细胞的超微结构和自体荧光特性的变化探讨了激光复壮藻种的可能机制.实验结果表明：辐照剂量为30 s~60 s时，有较好的复壮效果，藻种的生长速率、细胞干重、叶绿素、类胡萝卜素及蛋白质等含量均较对照组有不同程度的提高，增幅约25.3％～48.3％....
瞳孔检测仪的研究及其光学系统设计
医用光学 瞳孔检测仪 屈光手术
2009/8/13
研究了一种双眼瞳孔检测仪,进行了光学系统的设计和理论计算.采用低照度CCD摄像机摄取在近红外和可见光照明情况下的瞳孔像,利用形态学等算法处理图像,可对图像进行直径精确检测和动态分析.实验表明,该仪器在0~200 lux照度级下的测量精度可达0.01 mm,完全可以满足眼科手术的精度要求.
小麦生长过程中光能利用率和光化学反射指数的相关性研究
光化学反射指数 光能利用率 叶绿素
2009/8/13
以不同品种的小麦为材料，同时测量小麦生长过程的光谱反射率和光能利用率，研究了小麦整个生长期内光化学反射指数和光能利用率之间的关系变化，讨论二者之间关系的时间效应.实验结果表明，对小麦的整个生长期，光化学反射指数并不能作为估算光能利用率的一个有效指数.由于叶片叶绿素含量和叶面积指数的影响，在小麦的生长初期和末期，光化学反射指数和光能利用率之间的相关性不高，相关系数R2分别为0.222 4和0.210...
一种用于细胞操作的单光纤光镊研究
FDTD 医用光学 生物技术
2009/8/13
采用一种抛物线形光纤针作为基本器件，设计制作了单光纤光镊系统.用时域有限差分（Finite Difference Time Domain，FDTD）的方法仿真了抛物线型光纤探针的出射场，并在稳态场下通过对麦克斯韦应力张量积分求出不同介质球半径和折射率情况下，介质球受到的横向和纵向光作用力.实验中此单光纤光镊系统实现了对水中酵母细胞的空间捕获，并且结合两个抛物线形光纤针实现了酵母细胞在两个光势阱中的...
乙醇-水团簇分子发射荧光的机理研究
荧光光谱 电子能级结构 分子结构
2009/8/13
利用含时密度泛函理论分别计算了乙醇分子、水分子、以及乙醇和水形成的新团簇分子的基态和激发态能级，得出了各自吸收光能量和光波长的理论极限值；利用分析化学的理论分析了链式的乙醇-水团簇分子的特殊结构，得到了基态团簇分子间形成碰撞复合物的结论；利用量子力学的态叠加原理和量子化学的轨道理论，并结合Hckel近似对该复合物的能级和对应波函数进行了计算和推导，从而解释了乙醇-水溶液能吸收较长波长的光并发射荧光...
Characteristics of MIR-FEL Induced-Sound in Biological Tissues
MIR-FEL CO2 Laser Medical application
2009/6/15
In this paper, we examine the pressure level and the wave form, which is the sound produced when incising biological tissue using a TEA-CO2 laser and a Middle-Infrared Free Electron Laser (MIR-FEL). T...
Cholesterol Removal Using a MIR Free Electron Laser in an Atherosclerotic Region
MIR Free electron laser (FEL) Cholesterol ester
2009/6/15
In order to estimate the optimum laser conditions for efficient dissociation of cholesterol ester in an arterio-sclerotic region of blood vessels, we have investigated the relationship between the las...
Medical Application Research Using a Mid-Infrared Free Electron Laser
MIR-FEL Medical application Primary absorber
2009/6/15
A mid-infrared Free Electron Laser (MIR-FEL) has potentials for medical application research. The MIR-FEL has great two advantages: (1) tunability and (2) double pulse structure. The Osaka MIR-FEL is ...
Excitation Relaxation Dynamics of Carotenoids in Solutions and in Photosynthetic Pigment Protein Complexes
Photosynthetic system Carotenoid Excitation relaxation
2009/6/9
Excited state dynamics of carotenoids were investigated to access relationship among molecular structures, dynamics, and energy transfer to chlorophylls in photosynthetic pigment protein complexes. Th...
Energy Dissipation Processes of a Biological Protein in Condensed Phase
Energy relaxation Myoglobin Transient grating Heating
2009/6/9
There are many proteins that use photon energy as driving force for biological functions. The excess energy which was not used for the function ultimately flows into the thermal mode to heat up the sy...
The Ultrafast Photoresponce Dynamics and the Functions of the Electron Transfer Proteins
Coherent Plastocyanin Skeletal mode
2009/6/9
Recent revolutionary festinate development of the ultrafast laser technology enable us to take the snapshot of the molecular motion in real-time. These methods have been applied to the dynamics of the...
Ultrafast Photoinduced Reaction Dynamics and Coherent Oscillations in Photoresponsive Proteins
Ultrafast photoreaction Ultrafast Stokes shift Coherent vibration
2009/6/9
We have studied primary processes of ultrafast photoisomerization reactions of photoactive yellow protein (PYP) and visual rhodopsin (Rh) as well as ultrafast photoinduced electron transfer reactions ...
Preface to Special Issue on Pico/Femto-Second Molecular Dynamics in Biological Photoreceptors“Proteins-Complex Molecular Systems of Mystery”
Pico/Femto-Second Molecular Dynamics Biological Photoreceptors Proteins-Complex Molecular Systems of Mystery
2009/6/9
Preface to Special Issue on Pico/Femto-Second Molecular Dynamics in Biological Photoreceptors“Proteins-Complex Molecular Systems of Mystery”.
Laser Desorption/Ionization Time of Flight Mass Spectrometry Using a Free-Electron Laser
Free-electron laser (FEL) Laser desorption/ionization Time of flight mass spectrometry (TOFMS)
2009/6/9
This commentary article provides short accounts of matrix-assisted laser desorption/ionization (MALDI) and time of flight mass spectrometry (TOFMS), particularly perspective views of MALDI using an in...
Proteomics Research by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Proteomics MALDI MS Edman chemistry
2009/6/9
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) represents a well-accepted and reliable method for the characterization of proteins. The method has great advantages in terms...